Review



mouse anti-β-actin a3853  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore mouse anti-β-actin a3853
    Mouse Anti β Actin A3853, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc11430743-55-41-45
    Average 90 stars, based on 1 article reviews
    mouse anti-β-actin a3853 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Saline:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Incubation:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Western Blot:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Immunofluorescence:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Proximity Ligation Assay:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Luciferase:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.

    Transfection:

    Article Title: Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels
    Article Snippet: The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction LaboratoriesTM, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction LaboratoriesTM, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).

    Article Title: Pleiotropy of autism-associated chromatin regulators.
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Brain Ischemic Tolerance Triggered by Preconditioning Involves Modulation of Tumor Necrosis Factor-α-Stimulated Gene 6 (TSG-6) in Mice Subjected to Transient Middle Cerebral Artery Occlusion
    Article Snippet: Membranes were then pre-incubated in blocking buffer (5% non-fat milk in 0.05% Tween-20 TRIS-buffered saline) for 1 h at room temperature and then incubated overnight, at 4 °C, with the following primary antibodies: mouse anti-TSG-6 (1:1000; MABT108, Millipore, Milan, Italy) and mouse anti-β-actin (1:3000; A3853, Sigma-Aldrich), followed by the corresponding secondary antibodies (1:3000; Sigma-Aldrich) for 1 h at room temperature.

    Article Title: FBXL19 Targeted STK11 Degradation Enhances Cigarette Smoke-Induced Airway Epithelial Cell Cytotoxicity.
    Article Snippet: Thiazolyl Blue Tetrazolium Bromide (#M5655), leupeptin (CAS# 103476-89-7), MG132 (CAS# 133407-82-6), and anti-β-Actin antibody (#A3853) were obtained from Sigma (Carlsbad, CA).

    Article Title: Pleiotropy of autism-associated chromatin regulators
    Article Snippet: Chd2 antibody (1:1000, Novus Biologicals, NBP2-32563) and β-actin antibody (1:5000, Sigma-Aldrich, A3853) were detected using horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, G21234 and G21040) and ECL reaction (Bio-Rad, 1705062).

    Article Title: Amniotic fluid stem cell‐derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model
    Article Snippet: Several antibodies were used to detect extracellular vesicles standard markers: Alix (sc‐53540) 95 kDa, CD81 (sc‐166029) 22 kDa, Tsg101 (sc‐7964) 44 kDa, ARF6 (sc‐7971) 26 kDa (Santa Cruz Biotechnology), β‐Actin (A3853) 42 kDa, and LaminA (sigma‐Aldrich) 70 kDa.

    Article Title: Collagen density regulates tip–stalk cell rearrangement during angiogenesis via cellular bioenergetics
    Article Snippet: Membranes were then incubated overnight with primary antibodies, anti-glucose transporter GLUT1 antibody (Abcam, ab115730) and anti-β-Actin antibody (Millipore Sigma, A3853), at 4 °C.

    Article Title: Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1
    Article Snippet: The following antibodies were used: anti-MX1 [Cell Signaling Technologies (CST), Danvers, MA, USA; NBP1-33033], anti-IFIT1 (CST; 14769s), anti-STAT1 (CST; 9172s), anti-STAT1 (Abcam; AB210524 ), anti-p-STAT1 (CST; 9167s), anti-STAT2 (CST; 72604s), anti-p-STAT2 (CST; 88410s), anti-enterovirus D68 VP1 (GeneTex, Montreal, Canada; GTX132313), anti-HA (Thermo Fisher Scientific, Waltham, MA, USA; 71-5500), anti-Flag (Sigma Aldrich, St. Louis, MO, USA; F1804), anti-β-actin (Sigma; A3853), anti-α-tubulin (GenScript Biotech, Piscataway, NJ, USA; A01410), and anti-GAPDH (Sungene Biotech; KM9002). . Luciferase assay Plasmids containing the luciferase reporter gene were transfected into HEK293T cells.



    Similar Products

    86
    Merck & Co β actin a3853
    β Actin A3853, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/actin+anti+%CE%B2/pm41616836-54-4-9
    Average 86 stars, based on 1 article reviews
    β actin a3853 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Millipore mouse anti-β-actin a3853
    Mouse Anti β Actin A3853, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc11430743-55-41-45
    Average 90 stars, based on 1 article reviews
    mouse anti-β-actin a3853 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore β‐actin (a3853)
    β‐Actin (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc11156524-93-29-35
    Average 90 stars, based on 1 article reviews
    β‐actin (a3853) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore anti-β-actin antibody (#a3853)
    Anti β Actin Antibody (#A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pm38712759-50-12-18
    Average 90 stars, based on 1 article reviews
    anti-β-actin antibody (#a3853) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v)
    β Actin (Cat# A3853) Antibody, Bacterial Lps) From E. Coli O111:B4 (Cat# L4391, Lot 115m4090v), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pm35354645-47-10-26
    Average 90 stars, based on 1 article reviews
    β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore β-actin (a3853)
    β Actin (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc09881343-23-28-31
    Average 90 stars, based on 1 article reviews
    β-actin (a3853) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore mouse anti-β-actin (a3853)
    Mouse Anti β Actin (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc08700540-50-9-17
    Average 90 stars, based on 1 article reviews
    mouse anti-β-actin (a3853) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore β-actin (cat#: a3853) antibody
    A MLE12 cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and <t>β-actin</t> antibodies. B BEAS-2B cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. C Primary human small airway epithelial cells (HSAECs) were treated with LPS as indicated; cell lysate was collected and analyzed with PRMT4 and β-actin antibodies. For A – C , relative expression of PRMT4 was plotted in the below panels. D Lung tissue lysates from deidentified human lung samples from normal and infected patients were analyzed for PRMT4 and β-actin by immunoblotting. Densitometry was presented in the right panel. E PRMT4 mRNA levels were determined by qRT-PCR in LPS-treated bronchial epithelial cells. Experiments n = 3. Statistical significance was indicated with asterisk “*” between groups where P < 0.05 vs. control (0).
    β Actin (Cat#: A3853) Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc08414963-254-19-37
    Average 90 stars, based on 1 article reviews
    β-actin (cat#: a3853) antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore anti-β-actin-peroxidase (a3853)
    A MLE12 cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and <t>β-actin</t> antibodies. B BEAS-2B cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. C Primary human small airway epithelial cells (HSAECs) were treated with LPS as indicated; cell lysate was collected and analyzed with PRMT4 and β-actin antibodies. For A – C , relative expression of PRMT4 was plotted in the below panels. D Lung tissue lysates from deidentified human lung samples from normal and infected patients were analyzed for PRMT4 and β-actin by immunoblotting. Densitometry was presented in the right panel. E PRMT4 mRNA levels were determined by qRT-PCR in LPS-treated bronchial epithelial cells. Experiments n = 3. Statistical significance was indicated with asterisk “*” between groups where P < 0.05 vs. control (0).
    Anti β Actin Peroxidase (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2-actin+(a3853)/%CE%B2+actin+antibody/pmc07386624-167-3-20
    Average 90 stars, based on 1 article reviews
    anti-β-actin-peroxidase (a3853) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    A MLE12 cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. B BEAS-2B cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. C Primary human small airway epithelial cells (HSAECs) were treated with LPS as indicated; cell lysate was collected and analyzed with PRMT4 and β-actin antibodies. For A – C , relative expression of PRMT4 was plotted in the below panels. D Lung tissue lysates from deidentified human lung samples from normal and infected patients were analyzed for PRMT4 and β-actin by immunoblotting. Densitometry was presented in the right panel. E PRMT4 mRNA levels were determined by qRT-PCR in LPS-treated bronchial epithelial cells. Experiments n = 3. Statistical significance was indicated with asterisk “*” between groups where P < 0.05 vs. control (0).

    Journal: Cell Death & Disease

    Article Title: Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia

    doi: 10.1038/s41419-021-04115-7

    Figure Lengend Snippet: A MLE12 cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. B BEAS-2B cells were treated with LPS as indicated; cell lysates were immunoblotted with PRMT4 and β-actin antibodies. C Primary human small airway epithelial cells (HSAECs) were treated with LPS as indicated; cell lysate was collected and analyzed with PRMT4 and β-actin antibodies. For A – C , relative expression of PRMT4 was plotted in the below panels. D Lung tissue lysates from deidentified human lung samples from normal and infected patients were analyzed for PRMT4 and β-actin by immunoblotting. Densitometry was presented in the right panel. E PRMT4 mRNA levels were determined by qRT-PCR in LPS-treated bronchial epithelial cells. Experiments n = 3. Statistical significance was indicated with asterisk “*” between groups where P < 0.05 vs. control (0).

    Article Snippet: Cycloheximide (Cat#: ALX-380-269-G001, lot: 01061518) and Ubiquitin aldehyde (Cat#: BML-UW8450-0050, lot: 07021447) were from Enzo Life Sciences (Farmingdale, NY). β-Actin (Cat#: A3853) antibody and bacterial lipopolysaccharide (LPS) from E. coli O111:B4 (Cat#: L4391, lot: 115M4090V) were from Sigma (Carlsbad, CA).

    Techniques: Expressing, Infection, Western Blot, Quantitative RT-PCR

    A BEAS-2B cells were treated with cycloheximide (CHX), leupeptin, or MG132 as indicated. Cell lysates were immunoblotted with PRMT4 and β-actin antibodies. B Densitometry results in A were analyzed in semi-log format with GraphPad prism 5 and the results were plotted. C Ectopic expression of ubiquitin reduces PRMT4 protein in a concentration-dependent manner. Densitometry was plotted in the right panel. D PRMT4 is polyubiquitinated as shown by co-immunoprecipitation (Co-IP) of precipitates analyzed with ubiquitin and PRMT4 antibodies.

    Journal: Cell Death & Disease

    Article Title: Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia

    doi: 10.1038/s41419-021-04115-7

    Figure Lengend Snippet: A BEAS-2B cells were treated with cycloheximide (CHX), leupeptin, or MG132 as indicated. Cell lysates were immunoblotted with PRMT4 and β-actin antibodies. B Densitometry results in A were analyzed in semi-log format with GraphPad prism 5 and the results were plotted. C Ectopic expression of ubiquitin reduces PRMT4 protein in a concentration-dependent manner. Densitometry was plotted in the right panel. D PRMT4 is polyubiquitinated as shown by co-immunoprecipitation (Co-IP) of precipitates analyzed with ubiquitin and PRMT4 antibodies.

    Article Snippet: Cycloheximide (Cat#: ALX-380-269-G001, lot: 01061518) and Ubiquitin aldehyde (Cat#: BML-UW8450-0050, lot: 07021447) were from Enzo Life Sciences (Farmingdale, NY). β-Actin (Cat#: A3853) antibody and bacterial lipopolysaccharide (LPS) from E. coli O111:B4 (Cat#: L4391, lot: 115M4090V) were from Sigma (Carlsbad, CA).

    Techniques: Expressing, Concentration Assay, Immunoprecipitation, Co-Immunoprecipitation Assay

    A – C MLE12 cells, BEAS-2B cells, and human primary small airway epithelial cells (HSAECs) were treated with LPS as indicated. Cell lysates were analyzed by PRMT4, cleaved caspase 3, FBXO9, and β-actin immunoblotting. Shown below is densitometric analysis of immunoblots. D Overexpression of PRMT4 increases cleaved caspase 3, 8, and 9 baseline levels in BEAS-2B lung epithelial cells. E Knockdown of PRMT4 in BEAS-2B epithelial cells with shRNA reduces cleaved caspase 3, 8, and 9 expression. F , G Overexpression of PRMT4 enhances LPS-induced caspase 3 activation ( F ) and causes BEAS-2B lung epithelial cell death. Cell death is determined using LDH assay and the data are normalized with that from untreated control cells. H , I Silencing of PRMT4 inhibits LPS-induced caspase 3 activation ( H ) and cell death ( I ) in BEAS-2B cells. * P < 0.05. Experiments n = 3.

    Journal: Cell Death & Disease

    Article Title: Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia

    doi: 10.1038/s41419-021-04115-7

    Figure Lengend Snippet: A – C MLE12 cells, BEAS-2B cells, and human primary small airway epithelial cells (HSAECs) were treated with LPS as indicated. Cell lysates were analyzed by PRMT4, cleaved caspase 3, FBXO9, and β-actin immunoblotting. Shown below is densitometric analysis of immunoblots. D Overexpression of PRMT4 increases cleaved caspase 3, 8, and 9 baseline levels in BEAS-2B lung epithelial cells. E Knockdown of PRMT4 in BEAS-2B epithelial cells with shRNA reduces cleaved caspase 3, 8, and 9 expression. F , G Overexpression of PRMT4 enhances LPS-induced caspase 3 activation ( F ) and causes BEAS-2B lung epithelial cell death. Cell death is determined using LDH assay and the data are normalized with that from untreated control cells. H , I Silencing of PRMT4 inhibits LPS-induced caspase 3 activation ( H ) and cell death ( I ) in BEAS-2B cells. * P < 0.05. Experiments n = 3.

    Article Snippet: Cycloheximide (Cat#: ALX-380-269-G001, lot: 01061518) and Ubiquitin aldehyde (Cat#: BML-UW8450-0050, lot: 07021447) were from Enzo Life Sciences (Farmingdale, NY). β-Actin (Cat#: A3853) antibody and bacterial lipopolysaccharide (LPS) from E. coli O111:B4 (Cat#: L4391, lot: 115M4090V) were from Sigma (Carlsbad, CA).

    Techniques: Western Blot, Over Expression, shRNA, Expressing, Activation Assay, Lactate Dehydrogenase Assay